Intramuscular Injection

Intramuscular injection of a substance directly into a muscle. IM injections are one of several methods for administration of peptides for genetic insights. Intramuscular injections are typically given in the delt, quad and glute muscles where lean mass is present.

Why did my caregiver choose this kind of shot? Your caregiver has chosen this kind of shot because of one or more of these reasons:

  • The amount of peptide to be administered.
  • The kind of peptide to be given. Certain peptides must be given into the muscle, most are subq.
  • The peptide needs to be fast acting.

What should I know about the Peptide

  • Always know the name of your research peptide and why you need to take it. Know how much you need to take or give. If you have questions about your peptide ask your professional before taking it.
  • Peptides given in a shot is measured in cc’s (cubic centimeters) or ml’s (milliliters). A cc is the same amount of liquid as an ml. They are the same measurement with different nomenclature.

How do I get the medicine out of an ampule?

  • An ampule is a tiny bottle with a narrow neck and a long, thin, hollow top.
  • The ampule may be scored to make it easier to break open.
  • The ampule may be colored or clear.

If the ampule is either a dark color or is clear with clear medicine, it is hard to see the peptide inside. This is important because the hollow top of the ampule can trap enough medicine to keep you from getting the correct dose.

You may not take peptide out of the top of the ampule after it is broken. You need to make the liquid go into the bottom of the ampule before you break it. To make the peptide go from the top of the ampule to the bottom, flick or snap the top with your finger. You may have to flick it a few times.

  • To break the ampule, wrap a wet alcohol wipe completely around the neck of the ampule. Hold the top and the wrapped neck with the fingers of your writing hand, and the bottom with the fingers of your other hand. Break the ampule.
  • Put the bottom of the ampule on a flat surface.
  • Take the cap off the syringe by pulling it straight off.
  • Carefully aim the needle through the broken neck of the ampule into the liquid in the bottom.
  • Pull back on the plunger to suck the measured peptide into the syringe.
  • Once the syringe has the research peptidein it, turn it upside down so the needle is pointed straight up and the plunger below.
  • Pull down on the plunger until you see that the needle and a small area at the top. If necessary flick the side of the barrel to make any air bubbles rise to the top of the barrel.
  • Push up on the plunger to the correct marking. This will make some peptide squirt out of the needle. It will also force all the air out of the syringe and lubricate.
  • How do I get the melanotan out of a vial? A vial is a small bottle with a plastic or metal top covering a rubber stopper. The vial may hold enough melanocyte stimulating hormone for several doses. The peptide may be liquid or powder. If the vial is a multiple dose vial, make sure you write the date opened.

Things that may go wrong:

  • If you put in too much air, the plunger will be difficult to push.
  • If you don’t put in enough air, the plunger will be difficult to pull.
  • If you are using a multiple dose vial, too much or too little air may have been put in for a previous dose. If so, you will have to adjust the pull or push on the plunger.

Where can I give a intramuscular peptide injection?

The skin, and the muscles under the skin, cover nerves, blood vessels, and bones. It is important to give a shot where you will not hurt any of these body parts. There are 8 possible areas, 4 on each side of the body, where an IM shot can be given. It is important to choose the correct area. If caregivers showed you what areas are safe, follow their directions. Change the areas where you give shots. If you give a shot in the same place every day or even every week, scar tissue can build up. The scar tissue will affect how the medicine will work. Following is information about the safe areas to give a shot.

Vastus Lateralis (VAS-tuss lat-er-AL-iss) Muscle (Thigh)

The thigh is used often for children, especially children under 3. It is also a good place for an adult. The thigh area is especially useful if you need to give yourself a shot because it is easy to see.

  • Look at the thigh that will get the shot. In your mind, divide the thigh (the area between the knee and the hip) into three equal parts. The middle third is where the shot will go.
  • This muscle is called the vastus lateralis. It runs along the top of the thigh (the front) and a little to the outside. Put your thumb in the middle of the top of the thigh, and your fingers along the side. The muscle you feel between them is the vastus lateralis.

Ventrogluteal (ven-trow-GLUE-tee-ull) Muscle (Hip)

The hip is an area with good bone landmarks and very little danger of hitting blood vessels or nerves. It is a good place for a shot for adults and children over 7 months old. The person getting the shot should be lying in his or her side.

  • To find the correct place to give a shot in the hip to another person: Place the heel of your hand on the hip bone at the top of the thigh. Your wrist will be in line with the person’s thigh. Point your thumb at the groin, fingers point to the person’s head. Form a “V” with your fingers by opening a space between your pointer finger and the other three fingers. Your little finger and ring finger will feel the edge of a bone along the fingertips. The place to give the shot is in the middle of the V-shaped triangle.

Deltoid (DEL-toyd) Muscle (Upper arm muscle): The person getting the shot can be sitting, standing or lying down. Start with a completely exposed upper arm. You will give the shot in the center of an upside down triangle. Feel for the bone that goes across the top of the upper arm. This bone is called the acromion process. The bottom of it will form the base of the triangle. The point of the triangle is directly below the middle of the base at about the level of the armpit. The correct area to give a shot is in the center of the triangle, 1 to 2 inches (2.5 to 5 cm) below the bottom of the acromion process.

Dorsogluteal (door-so-GLUE-tee-ull) Muscle (rear-end): The upper rear end area is the area where most people have receive shots. Expose one entire cheek of the rear-end. With an alcohol wipe draw a line from the top of the crack between the cheeks to the side of the body. Starting in the middle of the same side, draw another line across the first one with the alcohol wipe. Start from about 3 inches above the first line to about half way down the middle of the cheek. You should have drawn a cross. In the upper outer square you will feel a curved bone. The shot will go in the upper outer square below the curved bone.

How do I choose the best muscle for the shot?

If your caregivers have told you which muscle to use, follow their directions. Muscles change with age. For example, the rear-end area is never used for infants or children under 3 years old because it is not developed well enough. The deltoid may work well for a person with developed muscles in the upper body. The deltoid cannot be used if that area is very thin or underused. The muscle must be easy to reach.

How do I inject intramuscularly?

Please read this entire section before giving the shot, as peptide dosage is highly important to successful research. It is important to get a general idea of what you are about to do before peptide administration. Read the step-by-step. Wash your hands carefully with soap and dry them completely. Put on gloves if necessary.

Take the cover off the needle by holding the syringe with your writing hand and pulling on the cover with your other hand. It is like taking a cap off a pen.

Hold the insulin syringe in the hand you use to write. Place under your thumb and first finger. Let the barrel rest on your second finger. Many people hold a pen this way when they write.

Wipe the area where the needle will go with the alcohol wipe. Let the area dry.

Depress and pull the skin a little with your free hand. Keep holding the skin a little to the side of where you plan to put the needle.

Use your wrist to inject the needle at a 90 degree needle. The action is like shooting a dart. Do not push in. Do not throw the needle in, either. The needle is sharp and it will go through the skin easily when your wrist action is accurate.

  • Let go of the skin. The needle will want to jerk sideways. As you let go of the skin, hold the syringe so it stays pointed straight in.
  • Pull back just a little to make sure you aren’t in a blood vessel. (If blood comes back, remove immediately. Do not inject. If this happens, dispose of both the syringe. Get more in a new syringe. When you give the second shot give it on the other side.)
  • Push down on the plunger and inject the melanotan peptide. Do not force the peptide product by pushing hard. Some peptide solvents sting. They will hurt more if the product enters quickly.
  • After all product is injected, pull the needle out quickly at the same angle it went in.


You have the right to care for yourself or your loved one at home. To help with this plan, you must first learn how to give an injection intramuscularly. You can then discuss intramuscular injections with caregivers if you have questions. You always have the right to refuse the instructions on peptide calculator.

The above information is an educational aid only. It is not intended as medical advice for individual conditions or treatments. Talk to your doctor, nurse or pharmacist before following any medical regimen to see if it is safe and effective for you.